1. Antibodies
  2. Homo sapiens + Mus musculus
  3. MAZ + Maz

ENCAB000AIM

Antibody against Homo sapiens MAZ, Mus musculus MAZ

Homo sapiens
HeLa-S3, GM12878, K562, MCF-7, A549
characterized to standards with exemption
Homo sapiens
any cell type or tissue
partially characterized
Mus musculus
any cell type or tissue
partially characterized
Homo sapiens
HepG2
not characterized to standards
Status
released
Source (vendor)
Abcam
Product ID
ab85725
Lot ID
GR41711-2
Host
rabbit
Clonality
polyclonal
Purification
affinity
Antigen description
Immunogen is synthetic peptide, corresponding to a region within amino acids 427-477 of Human MAZ, GenBank BAA33064.1.

Characterizations

MAZ (Homo sapiens)
Method: immunoprecipitation
Attachment PDF Icon
not reviewed
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
MAZ (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
not reviewed
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
MAZ (Homo sapiens)
HeLa-S3
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
B. Immunoprecipitation of Maz from HeLa-S3 cells using ab85725. Lane 1: input nuclear lysate. Lane 2: unbound material from immunoprecipitation with ab87525. Lane 3: material immunoprecipitated with ab85725. Lane 4: material immunoprecipitated using control IgG. Arrows indicate band at expected size of Maz, and * indicates band of similar intensity and unexpected size (identified as Maz by mass spectrometry).
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
MAZ (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation of Maz from K562 cells using ab85725. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with ab85725. Lane 3: material immunoprecipitated using control IgG. Bands A and B were excised from the gel and subject to analysis by mass spectrometry.
Reviewer comment
What is the expected band size?
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
MAZ (Homo sapiens)
A549
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: A549 using the antibody ab85725. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 48.608.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
 MAZ_A549.jpg
MAZ (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
exempt from standards
Caption
IP followed by mass spectrometry: Briefly, K562 whole cell lysates were immunoprecipitated using ab85725, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGE Bis-Tris Gel) and separated with an invitrogen NuPAGE electrophoresis system. The gel was silver-stained, gel fragments corresponding to the bands indicated were excised and destained using the SilverSNAP Stain for Mass Spectrometry (Pierce). Then proteins were treated with trypsin using the in-gel digestion method. Digested proteins were analyzed on an LTQ-Orbitrap (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (protein false discovery rate <1%, 2 peptides per protein minimum).
Submitter comment
Though not at expected size, IP-MS still identifies protein of interest enriched in immunoreactive bands
Reviewer comment
MAZ is not within the top 20 ranked by fold enrichment in band A (signal at expected size) but is for band B (not at expected size).
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
MAZ (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7 using the antibody ab85725. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 48.608.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
 MAZ_MCF7.jpg
MAZ (Mus musculus)
Method: immunoprecipitation
Attachment PDF Icon
not reviewed
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
MAZ (Mus musculus)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
not reviewed
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
MAZ (Homo sapiens)
GM12878K562HeLa-S3HepG2
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
A. Western blot using ab85724. Lanes contain nuclear lysates from GM12878 cells (lane 1), K562 cells (lane 2), HeLa-S3 cells (lane 3), and HepG2 cells (lane 4). Expected size: ~48.6kD
Reviewer comment
May be exempt from standard if IP-MS is deemed compliant.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford