ENCAB000AHT
Antibody against Homo sapiens SUPT5H
Homo sapiens
at least one cell type or tissue
awaiting characterization
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-28678
- Lot ID
- unknown
- Characterized targets
- SUPT5H (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- Protein A/G
- Antigen description
- SPT5 Antibody (H-300) epitope corresponding to amino acids 61-360 mapping near the N-terminus of SPT5 of human origin.
- External resources
Characterizations
SUPT5H (Homo sapiens)
Method: immunoblot
not reviewed
- Submitted by
- Kevin Struhl
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG004558
SUPT5H (Homo sapiens)
Method: immunoprecipitation
not reviewed
- Caption
- Analysis of Spt5 ChIP-Seq. confirms specificity of ChIP. A 34% of Spt5 ChIP-Seq peaks (p-value < 1e-10) are found within 1000bp of a RefSeq transcriptional start site (TSS), consistent with the role of Spt5 in blocking RNA polymerase II after early elongation, the majority of peaks are found downstream of the TSS. B Spt5 peaks overlap directly with cognate negative elongation factor, NELFe, and RNAPII, and downstream of the general transcription factors TFIIB and TBP. C DSIF (Spt4/Spt5) is known to act proximal to the transcriptional start site and travel with elongating RNAPII. Consistent with a role downstream of the start site a significant number of Spt5 ChIP-Seq. peaks are found across RefSeq genes and proximal to the transcriptional end site, compared to NELFe which associates specifically with transcriptional start sites.
- Submitted by
- Kevin Struhl
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG004558