ENCAB000AHS

Antibody against Homo sapiens HSF1

Homo sapiens
HepG2, MCF-7, GM12878
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-9144
Lot ID
unknown
Characterized targets
HSF1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Isotype
IgG
Antigen description
Epitope corresponding to amino acids 219-529 of heat shock transcription factor 1 (HSF1) of human origin

Characterizations

HSF1 (Homo sapiens)
Method: motif enrichment
compliant
Caption
The motif for target HSF1 is represented by the attached position weight matrix (PWM) derived from ENCFF085MNM. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.959238937087 Global enrichment Z-score: 7.99131567315 Positional bias Z-score: 11.7447269585 Peak rank bias Z-score: 8.54940658081 Enrichment rank: 1.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
HSF1 (Homo sapiens)
Method: motif enrichment
compliant
Caption
The motif for target HSF1 is represented by the attached position weight matrix (PWM) derived from ENCFF327WUS. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.959238937087 Global enrichment Z-score: 8.57808629438 Positional bias Z-score: 8.18030189771 Peak rank bias Z-score: 7.94509540733 Enrichment rank: 1.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
HSF1 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody sc-9144. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 80.0
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
HSF1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody sc-9144. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 80.0
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
HSF1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
not reviewed
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from GM12878 nuclear cell lysates using the antibody sc-9144, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
None of the proteins ranked above or equal to HSF1 are sequence-specific TFs.
Reviewer comment
Not reviewed because this antibody has already been fully characterized in GM12878.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
HSF1 (Homo sapiens)
Method: immunoprecipitation
not reviewed
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
HSF1 (Homo sapiens)
Method: motif enrichment
not reviewed
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
HSF1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
B. Immunoprecipitation of HSF1 from forskolin-stimulated HepG2 cells using sc-9144. Lane 1: input nuclear lysate, Lane 2: unbound material from immunoprecipitation with sc-9144, Lane 3: material immunoprecipitated with sc-9144, Lane 4: material immunoprecipitated using control IgG. The expected band size is 70 kD, indicated by the arrow.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
HSF1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
not reviewed
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line GM12878 using the antibody sc-9144. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 80.0.
Reviewer comment
Not reviewed because this antibody has already been fully characterized in GM12878.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford