ENCAB000AHR
Antibody against Homo sapiens CBX3
Homo sapiens
K562, HepG2, GM12878, SK-N-SH
characterized to standards
Homo sapiens
A549, H1
not characterized to standards
- Status
- released
- Source (vendor)
- Abcam
- Product ID
- ab10480
- Lot ID
- GR22075-1
- Characterized targets
- CBX3 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Antigen description
- Synthetic peptide conjugated to KLH, derived from within residues 1 - 100 of Mouse HP1 gamma
- Aliases
- bradley-bernstein:PchAb 362
- External resources
Characterizations
CBX3 (Homo sapiens)
Method: ChIP-seq comparison
compliant
- Submitter comment
- SAV3
- Submitted by
- Nina Farrell
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- CBX3 PchAb 362 SAV.pdf
CBX3 (Homo sapiens)
K562HepG2GM12878SK-N-SH
Method: immunoblot
compliant
- Caption
- Nuclear lysates from K562 (10ug), HepG2 (7ug), GM12878 (7ug), SK-N-SH (6ug), were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. a single band of the expected size was detected (~24kDa).
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
CBX3 (Homo sapiens)
A549H1
Method: immunoblot
not compliant
- Caption
- Nuclear lysates from A549 (10ug) and H1 (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Bands were detected at the expected size (24kDa) and also at 50kDa and 60kDa.
- Reviewer comment
- must be at least 50% of the signal at 20kDa
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
CBX3 (Homo sapiens)
Method: ChIP-string comparison
not reviewed
- Caption
- Antibody was validated by use of correlation analysis of ChIP-String data obtained using this antibody together with diverse other antibodies
- Submitted by
- Bradley Bernstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG004570
CBX3 (Homo sapiens)
K562
Method: immunoblot
compliant
- Submitted by
- Bradley Bernstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG004570