ENCAB000AHR

Antibody against Homo sapiens CBX3

Homo sapiens
K562, SK-N-SH, GM12878, HepG2
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
A549, H1-hESC
not characterized to standards
Status
released
Source (vendor)
Abcam
Product ID
ab10480
Lot ID
GR22075-1
Host
rabbit
Clonality
polyclonal
Purification
affinity
Antigen description
Synthetic peptide conjugated to KLH, derived from within residues 1 - 100 of Mouse HP1 gamma
Aliases
bradley-bernstein:PchAb 362

Characterizations

CBX3 (Homo sapiens)
Method: ChIP-seq comparison
compliant
Submitter comment
SAV3
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad
CBX3 (Homo sapiens)
K562HepG2GM12878SK-N-SH
Method: immunoblot
Attachment from submitter
compliant
Caption
Nuclear lysates from K562 (10ug), HepG2 (7ug), GM12878 (7ug), SK-N-SH (6ug), were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. a single band of the expected size was detected (~24kDa).
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
CBX3 (Homo sapiens)
A549H1-hESC
Method: immunoblot
Attachment from submitter
not compliant
Caption
Nuclear lysates from A549 (10ug) and H1 (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Bands were detected at the expected size (24kDa) and also at 50kDa and 60kDa.
Reviewer comment
must be at least 50% of the signal at 20kDa
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
CBX3 (Homo sapiens)
Method: ChIP-string comparison
not reviewed
Caption
Antibody was validated by use of correlation analysis of ChIP-String data obtained using this antibody together with diverse other antibodies
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad
CBX3 (Homo sapiens)
Method: immunoblot
not reviewed
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad