ENCAB000AGZ

Antibody against Homo sapiens GATA3

Homo sapiens
any cell type or tissue
awaiting characterization
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-269
Lot ID
A1404
Characterized targets
GATA3 (Homo sapiens)
Host
mouse
Clonality
monoclonal
Isotype
IgG
Antigen description
Raised against human recombinant GATA-3 (amino acids 1-264).

Characterizations

GATA3 (Homo sapiens)
Method: knockdown or knockout
not reviewed
Caption
GATA3 siRNA transfection and western blotting: 20 nM siRNA targeting GATA3 or non-specific control siRNA (siCONTROL) were transfected into MCF-7 cells using lipofectamine for 72 hours. 15 ug nuclear extracts were loaded for SDS-PAGE and western blotting using anti-GATA3 antibodies (1ug/mL). Actin was used as a loading control, the same membrane was stripped and reprobed with anti-Actin antibodies (0.1ug/mL).
Submitted by
Peggy Farnham
Lab
Michael Snyder, Stanford
GATA3 (Homo sapiens)
Method: ChIP-seq comparison
not reviewed
Caption
ChIP-seq was performed using a GATA3 antibody in SHSY5Y cells. The top 1000 peaks were analyzed with a de novo motif analysis (http://motif.bmi.ohio-state.edu/ChIPMotifs/). The top motif that was identified was TTATCT (P value: 1.965e-319), which in the reverse direction is AGAGAA. A previously identified motif for GATA1 using ChIP-seq data is WGATAR (Mol Cell. 36:667-681, 2009), which is essentially the same as the new de novo motif identified for GATA3. We then analyzed the percentage of peaks containing the de novo GATA3 motif in the entire set of GATA3 called peaks. We found that 78% of the top 1000 peaks contained this motif, with the % dropping to 70% in the top 5000 and to 60% in the entire dataset.
Submitted by
Peggy Farnham
Lab
Michael Snyder, Stanford