ENCAB000AGX
Antibody against Mus musculus GATA2
Mus musculus
at least one cell type or tissue
awaiting characterization
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-9008
- Lot ID
- J2108
- Characterized targets
- GATA2 (Mus musculus)
- Host
- rabbit
- Clonality
- polyclonal
- Antigen description
- Raised against amino acids 120-235 of GATA-2 of human origin.
- External resources
Characterizations
GATA2 (Mus musculus)
Method: immunoblot
not reviewed
- Caption
- Whole cell extracts prepared from G1E, G1E-ER4+E2, MEL, and CH12 cells were separated by SDS-PAGE and transferred to PVDF using standard immunoblotting methods. GATA2 expression was detected using rabbit anti-GATA2 (H-116; sc-9008, 1:5000) followed by incubation with anti-rabbit secondary antibody (1:5000) and detected by ECL Plus (AmershamBiosciences). As expected, GATA2 was detected in G1E cells (a model for erythroid progenitor cells), but not G1E-ER4+E2 cells (a model for differentiatingerythroblasts), consistent with published microarray data (Welch et al., 2004) showing that the Gata2 gene is expressed abundantly in progenitors but is strongly repressed during differentiation.As expected, no GATA2 is detected in MEL (erythroblast model) or CH12 cells (a B-cell lymphoma; GATA2 is not found in this lineage). Thus the specificity of this antibody is shown by the detection of a single band in G1E cells and the loss of that signal upon repression of the Gata2 gene.
- Submitted by
- Ross Hardison
- Lab
- Ross Hardison, PennState
- Grant
- RC2HG005573