ENCAB000AGS

Antibody against Homo sapiens GATA1

Homo sapiens
at least one cell type or tissue
awaiting characterization
Status
released
Source (vendor)
Emery Bresnick
Product ID
GATA-1
Lot ID
unknown
Characterized targets
GATA1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
other
Antigen description
Antibody generated using a purified GST fusion to amino acids 1-89 of mouse GATA-1.

Characterizations

GATA1 (Homo sapiens)
Method: immunoblot
Attachment from submitter
not reviewed
Caption
GATA-1 western blot. 10 ug of K562 nuclear extract was run on an acrylamide gel and probed with the GATA-1 antibody. One band of the correct size was detected.
Submitted by
Peggy Farnham
Lab
Michael Snyder, Stanford
GATA1 (Homo sapiens)
Method: ChIP-seq comparison
Attachment from submitter
not reviewed
Caption
Quantitative ChIP analysis of GATA factor occupancy at GATA-1 targets which were identified in K562 cells was performed in b-estradiol-untreated and -treated (1 mM, 24 hr) G1E-ER-GATA-1 cells (mean ± SE, three independent experiments). The black bars show that the antibody produces a robust ChIP signal only after induction of GATA-1 by treatment with hormone. See Molecular Cell 36, 667-681, 2009.
Submitted by
Peggy Farnham
Lab
Michael Snyder, Stanford