ENCAB000AGJ
Antibody against Homo sapiens FOSL1
Homo sapiens
at least one cell type or tissue
awaiting characterization
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-183
- Lot ID
- I0809
- Characterized targets
- FOSL1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Isotype
- IgG
- Antigen description
- Epitope mapping at the N-terminus of FOSL1 of human origin.
- External resources
Characterizations
FOSL1 (Homo sapiens)
not reviewed
- Caption
- Western blot protocol: Whole cell lysate or nuclear extract was immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed on an Invitrogen BenchPro 4100 system. Visualization was achieved using SuperSignal West Femto solution (Thermo Scientific). Results: Band of expected size visualized, representing strongest signal in the lane. Figure legend: IP-western with sc-183 in whole cell lysates (WCL) or nuclear extract (NE) of K562 and HeLa; PM=protein marker. FOSL1 band is indicated, as is heavy chain of IgG.
- Submitted by
- Richard Myers
- Lab
- Richard Myers, HAIB
- Grant
- U54HG004576