ENCAB000AGJ

Antibody against Homo sapiens FOSL1

Homo sapiens
at least one cell type or tissue
awaiting characterization
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-183
Lot ID
I0809
Characterized targets
FOSL1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Isotype
IgG
Antigen description
Epitope mapping at the N-terminus of FOSL1 of human origin.

Characterizations

FOSL1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
not reviewed
Submitted by
Richard Myers
Lab
Richard Myers, HAIB
FOSL1 (Homo sapiens)
Method: immunoblot
not reviewed
Caption
Western blot protocol: Whole cell lysate or nuclear extract was immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed on an Invitrogen BenchPro 4100 system. Visualization was achieved using SuperSignal West Femto solution (Thermo Scientific). Results: Band of expected size visualized, representing strongest signal in the lane. Figure legend: IP-western with sc-183 in whole cell lysates (WCL) or nuclear extract (NE) of K562 and HeLa; PM=protein marker. FOSL1 band is indicated, as is heavy chain of IgG.
Submitted by
Richard Myers
Lab
Richard Myers, HAIB