1. Antibodies
  2. Homo sapiens
  3. ELK1

ENCAB000AGB

Antibody against Homo sapiens ELK1

Homo sapiens
MCF-7, K562, IMR-90, A549
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
Epitomics
Product ID
1277-1
Lot ID
YG030402C
Characterized targets
ELK1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Antigen description
Synthetic peptide corresponding to residues in C-terminus of human ELK-1 was used as immunogen. The antibody does not cross-react with other ETS family members.

Characterizations

ELK1 (Homo sapiens)
A549
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: A549, using the antibody 1277-1. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 62.0
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ELK1 (Homo sapiens)
IMR-90IMR-90
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: IMR90, using the antibody 1277-1. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 62.0
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
ELK1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody 1277-1. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
Download
 811 1_ ELK1.jpg
ELK1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 whole cell lysates using 1277-1, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGE Bis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was silver-stained, gel fragments corresponding to the bands indicated were excised and destained using the SilverSNAP Stain for Mass Spectrometry (Pierce). Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an LTQ-Orbitrap (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Protein false discovery rate < 1%, 2 peptides per protein minimum).
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
ELK1 (Homo sapiens)
Method: motif enrichment
Attachment PDF Icon
compliant
Caption
The motif for target ELK1 is represented by the attached position weight matrix (PWM) derived from ENCFF732WQJ. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab) using known motifs (http://compbio.mit.edu/encode-motifs/) and previously published ChIP-seq data (http://www.broadinstitute.org/~zzhang/motifpipeline/data/TrainSetInfo.txt). The accept probability score of the given transcription factor was calculated using a Bayesian approach. This analysis also includes three motif enrichment scores, computed by overlapping the motif instances with the given ChIP-seq peak locations, as well as an enrichment rank. For more information on the underlying statistical methods, please see the attached document. Accept probability score: 0.857103478 Global enrichment Z-score: 3.305428989 Positional bias Z-score: 3.365156281 Peak rank bias Z-score: 4.004685043 Enrichment rank: 3.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
ELK1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
not reviewed
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 whole cell lysates using 1277-1, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGE Bis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was silver-stained, gel fragments corresponding to the bands indicated were excised and destained using the SilverSNAP Stain for Mass Spectrometry (Pierce). Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an LTQ-Orbitrap (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Protein false discovery rate < 1%, 2 peptides per protein minimum).
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
ELK1 (Homo sapiens)
Method: immunoprecipitation
Attachment PDF Icon
not reviewed
Caption
We observe one major band of ~65kD in all cell lines assayed (Panel A). This band is immunoprecipitated from K562 cells at very high efficiency and is absent in an IgG control immunoprecipitation. (Panel B and second validation assay). The major band migrates somewhat slower than predicted for ELK1 (~44kD), but this is generally observed with ELK1 (see product literature for this and other commercially available antibodies). Moreover, this band was confirmed to contain ELK1 by mass spectrometry (see second validation assay). Therefore, 1277-1 meets this criterion for validation.
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
ELK1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation of ELK1 from K562 cells using 12777-1. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with 1277-1. Lane 3: material immunoprecipitated using control IgG. Band A was excised from gel and subject to analysis by mass spectrometry.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford