1. Antibodies
  2. Homo sapiens
  3. ELF1

ENCAB000AGA

Antibody against Homo sapiens ELF1

Homo sapiens
at least one cell type or tissue
awaiting characterization
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-631
Lot ID
L2707
Characterized targets
ELF1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Isotype
IgG
Antigen description
Epitope mapping at the C-terminus of Elf-1 of human origin.

Characterizations

ELF1 (Homo sapiens)
Method: immunoblot
Attachment PDF Icon
not reviewed
Caption
Western blot protocol: Whole cell lysate was immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed on an Invitrogen BenchPro 4100 system. Visualization was achieved using SuperSignal West Femto solution (Thermo Scientific). Results: Band of expected size visualized, representing strongest signal in the lane. Figure legend: IP-western with sc-631 in whole cell lysate of GM12878, HeLa, and K562. ELF1 bands are indicated, as are heavy and light chains of IgG.
Submitted by
Richard Myers
Lab
Richard Myers, HAIB
ELF1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
not reviewed
Caption
IP followed by mass spectrometry: Briefly, GM12878 whole cell lysates were immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. Gel was stained with Coomassie Blue in order to visualize marker bands. Gel fragments corresponding to the bands indicated above in the western blot image were excised and sent to the University of Alabama at Birmingham Cancer Center Mass Spectrometry/Proteomics Shared Facility. There the samples were run on an LTQ XL Linear Ion Trap Mass Spectrometer by LC-ESI-MS/MS. Peptides were identified using MASCOT tandem mass spectra analysis, with probability based matching at p < 0.05. As per ENCODE data standards, all MASCOT results are attached, including common contaminants. Target protein is listed as hit 16 for lower band and hit 6 for upper band (targets are highlighted in bold font).
Submitted by
Richard Myers
Lab
Richard Myers, HAIB