1. Antibodies
  2. Homo sapiens
  3. EBF1

ENCAB000AFX

Antibody against Homo sapiens EBF1

Homo sapiens
at least one cell type or tissue
awaiting characterization
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-137065
Lot ID
J0505
Characterized targets
EBF1 (Homo sapiens)
Host
mouse
Clonality
monoclonal
Isotype
IgG
Antigen description
Raised against a.a. 1-300 mapping at the N-terminus of EBF of human origin.

Characterizations

EBF1 (Homo sapiens)
Method: immunoprecipitation
Attachment PDF Icon
not reviewed
Caption
Whole cell lysates were immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed on an Invitrogen BenchPro 4100 system. Visualization was achieved using SuperSignal West Femto solution (Thermo Scientific).
Submitted by
Richard Myers
Lab
Richard Myers, HAIB
EBF1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
not reviewed
Caption
Whole cell lysates were immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Blotting with primary (sameas that used for IP) and secondary HRP-conjugated antibodies was performed on an Invitrogen BenchPro 4100 system. Visualization was achieved using SuperSignalWest Femto solution (Thermo Scientific). Figure Legend: EBF immunoblot: IP-western with sc-137065EBF antibody in whole cell lysates (WCL) of K562 and GM12878.Heavy chain and light chain of IgG are indicated, and EBF band isindicated at ~64 kDa.
Submitted by
Richard Myers
Lab
Richard Myers, HAIB