ENCAB000AFV
Antibody against Homo sapiens E2F4, Mus musculus E2F4
Homo sapiens
at least one cell type or tissue
awaiting characterization
Mus musculus
at least one cell type or tissue
awaiting characterization
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-866
- Lot ID
- unknown
- Characterized targets
- E2F4 (Homo sapiens), E2F4 (Mus musculus)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Antigen description
- Epitope mapping at the C-terminus of E2F4 of human origin
- External resources
Characterizations
E2F4 (Homo sapiens)
not reviewed
- Caption
- A. An IP western peforemed using Gm12878 extract. The E2F4 antibody recognized one band of the right size in nuclerar extract 9lane 1 ). this band was immunoprecipitated by the E2F4 antibody (lane2) but not by a control rabbit IgG (lane3). B. ChIPs were performed with E2F4 and Rabbit IgG antibodies in K562 cells. Samples were alyzed by PCR using primers to known E2F4 targets. The target sites were precipitated by the E2F4 antibody but not by the control IgG. The negative control site (ZNF333) was not precipitated by the E2F4 antibody.
- Submitted by
- Peggy Farnham
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG004558
- Download
- human_E2F4_validation_Farnham.pdf
E2F4 (Homo sapiens)
not reviewed
- Caption
- a. An IP western was performed using Gm12878 extract. The E2F4 antibody recognized one band of the ight size in nuclear extract (lane10. This band was immunoprecipitated by the E2F4 antibody but not by the control IgG. The negative control site (ZNF333) was not precipitated by th E2F4 antibody.
- Submitted by
- Peggy Farnham
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG004558
- Download
- human_E2F4_validation_Farnham.pdf
E2F4 (Mus musculus)
not reviewed
- Caption
- This antibody has been validated by the Farnham lab by ChIP-Seq data in human cell lines. Please see the validation document for human cell lines for details.
- Submitted by
- Michael Snyder
- Lab
- Michael Snyder, Stanford
- Grant
- RC2HG005602
- Download
- mouse_E2F4_validation_Snyder.pdf
E2F4 (Mus musculus)
not reviewed
- Caption
- Immunoprecipitation of CH12 and MEL nuclear extracts using anti-E2F4 antibody (sc-866) enriched a band of the expected molecular weight of E2F4 (~60kD).
- Submitted by
- Michael Snyder
- Lab
- Michael Snyder, Stanford
- Grant
- RC2HG005602
- Download
- mouse_E2F4_validation_Snyder.pdf