ENCAB000AFV

Antibody against Mus musculus E2F4, Homo sapiens E2F4

Mus musculus
at least one cell type or tissue
awaiting characterization
Homo sapiens
at least one cell type or tissue
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-866
Lot ID
unknown
Host
rabbit
Clonality
polyclonal
Purification
affinity
Antigen description
Epitope mapping at the C-terminus of E2F4 of human origin

Characterizations

E2F4 (Homo sapiens)
Method: immunoprecipitation
not reviewed
Caption
A. An IP western peforemed using Gm12878 extract. The E2F4 antibody recognized one band of the right size in nuclerar extract 9lane 1 ). this band was immunoprecipitated by the E2F4 antibody (lane2) but not by a control rabbit IgG (lane3). B. ChIPs were performed with E2F4 and Rabbit IgG antibodies in K562 cells. Samples were alyzed by PCR using primers to known E2F4 targets. The target sites were precipitated by the E2F4 antibody but not by the control IgG. The negative control site (ZNF333) was not precipitated by the E2F4 antibody.
Submitted by
Peggy Farnham
Lab
Michael Snyder, Stanford
E2F4 (Homo sapiens)
Method: ChIP-seq comparison
not reviewed
Caption
a. An IP western was performed using Gm12878 extract. The E2F4 antibody recognized one band of the ight size in nuclear extract (lane10. This band was immunoprecipitated by the E2F4 antibody but not by the control IgG. The negative control site (ZNF333) was not precipitated by th E2F4 antibody.
Submitted by
Peggy Farnham
Lab
Michael Snyder, Stanford
E2F4 (Mus musculus)
Method: immunoprecipitation
not reviewed
Caption
This antibody has been validated by the Farnham lab by ChIP-Seq data in human cell lines. Please see the validation document for human cell lines for details.
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
E2F4 (Mus musculus)
Method: ChIP-seq comparison
not reviewed
Caption
Immunoprecipitation of CH12 and MEL nuclear extracts using anti-E2F4 antibody (sc-866) enriched a band of the expected molecular weight of E2F4 (~60kD).
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford