1. Antibodies
  2. Homo sapiens
  3. CTCFL

ENCAB000AFS

Antibody against Homo sapiens CTCFL

Homo sapiens
K562
not characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-98982
Lot ID
E0109
Characterized targets
CTCFL (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Isotype
IgG
Antigen description
Epitope corresponding to amino acids 91-210 mapping within an internal region of BORIS(CTCFL) of human origin.

Characterizations

CTCFL (Homo sapiens)
K562
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
Increasing amounts of K562 whole cell lysate were separated on a 12% acrylamide gel with the Bio-Rad PROTEAN II xi system.After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system.The membrane was probed with primary antibody (sc-98982; anti-CTCFL) and a secondary HRP-conjugated antibody.The resulting bands were visualized with SuperSignal West Femto solution (Thermo Scienfiic). Results & Figure legend: CTCFL (sc-98982) immunoblot in K562 cells.Protein marker (PM) labeled in kDa.Bands of expected sizes for CTCFL isoforms (~96 and 80 kDa) were detected, representing the only signals in the blot.
Submitted by
Flo Pauli-Behn
Lab
Richard Myers, HAIB
CTCFL (Homo sapiens)
Method: ChIP-seq comparison
Attachment from submitter
Attachment from submitters
not compliant
Caption
ChIP-seq was performed with an antibody (Active Motif, 39852) that targets a different part of the CTCFL protein in two replicates of K562 cells.CTCFL occupancy sites were identified with the MACS peak caller.These sites were compared with IDR (Landt et al. 2012, Li et al. 2011, https://sites.google.com/site/anshulkundaje/projects/idr) to the CTCFL occupancy sites that were previously identified in two replicates of K562 cells using the sc-98982 antibody. Results:IDR results of ChIP comparison by IDR between two K562 biological replicates of ChIP with CTCFL (sc-98982) and CTCFL (AM 39852).Most of the Np/Nt and N1/N2 ratios are less than 2, indicating significant replication of binding sites when ChIP is performed with the two antibodies directed against CTCFL.
Submitted by
Flo Pauli-Behn
Lab
Richard Myers, HAIB