ENCAB000AFR

Antibody against Homo sapiens CTCF

Homo sapiens
K562, HeLa-S3, GM12878
not characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-5916
Lot ID
K0902
Characterized targets
CTCF (Homo sapiens)
Host
goat
Clonality
polyclonal
Isotype
IgG
Antigen description
Epitope mapping at the N-terminus of CTCF of human origin.

Characterizations

CTCF (Homo sapiens)
K562GM12878HeLa-S3
Method: immunoblot
Attachment from submitter
compliant
Caption
Whole cell lysate or nuclear extract were immunoprecipitated using primary antibody.The IP fraction was separated on a 12% acrylamide gel with the Bio-Rad PROTEAN II xi system.After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system.The membrane was probed with primary antibody (same as that used for IP) and a secondary HRP-conjugated antibody.The resulting bands were visualized with SuperSignal West Femto solution (Thermo Scienfiic). Results & Figure legend: IP-western with sc-5916 anti-CTCF antibody in nuclear extract (NE) of K562 and whole cell lysate (WCL) of K562, GM12878, and HeLa cells.Protein marker (PM) labeled in kDa.A band of expected size for CTCF (~120 kDa) was detected, representing the strongest signal, except for heavy chain contamination.
Submitter comment
no IgG control
Submitted by
Flo Pauli-Behn
Lab
Richard Myers, HAIB
CTCF (Homo sapiens)
Method: ChIP-seq comparison
Attachment from submitter
not compliant
Caption
ChIP-seq was performed with an antibody (Active Motif, 39852) that targets a different part of the CTCF protein in two replicates of K562 cells.CTCF occupancy sites were identified with the MACS peak caller.These sites were compared with IDR (Landt et al. 2012, Li et al. 2012, https://sites.google.com/site/anshulkundaje/projects/idr) to the CTCF occupancy sites that were previously identified in two replicates of K562 cells using the sc-5916 antibody. Results:IDR results of ChIP comparison by IDR between two K562 biological replicates of ChIP with CTCF (sc-5916) and CTCF (AM 61312).All of the Np/Nt ratios are less than 2, indicating significant replication of binding sites when ChIP is perfomed with the two antibodies directed against CTCF.
Reviewer comment
The comparison ChIP data is not submitted nor is the antibody characaterized
Submitted by
Flo Pauli-Behn
Lab
Richard Myers, HAIB