ENCAB000AFM
Antibody against Homo sapiens CREB1
Homo sapiens
at least one cell type or tissue
awaiting characterization
- Status
- released
- Source (vendor)
- Cell Signaling
- Product ID
- 9198
- Lot ID
- unknown
- Characterized targets
- CREB1 (Homo sapiens)
- Host
- rabbit
- Clonality
- monoclonal
- Purification
- Protein A/G
- Antigen description
- Synthetic phosphopeptide corresponding to residues surrounding Ser133 of human CREB.
- External resources
Characterizations
CREB1 (Homo sapiens)
Method: ChIP-seq comparison
not reviewed
- Caption
- pS133-CREB ChIP-Seq analysis supports successful ChIP. A 36% of MACS-called ChIP-Seq peaks are found within 1000bp of a known TSS. pS133-CREB peaks preferentially overlap with CREB, ATF1, and ATF2 ChIP-Seq peaks compared to other transcription factors. B De novo motif discovery with the MEME suite of analysis software using 100bp of sequence flanking the top 1000 pS133-CREB peaks identifies the CRE palindrome as an enriched DNA element (p-value < 3e-3). 26% of all pS133-CREB peaks have a CRE (p-value < 0.0001) within 200bp, significantly higher than the 8.6% of non-redundant RefSeq TxStart sites (p-valuebonf < 1e-280). pS133-CREB peak and CRE position are strongly correlated.
- Submitted by
- Kevin Struhl
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG004558
CREB1 (Homo sapiens)
Method: immunoblot
not reviewed
- Caption
- Western blot analysis finds a single major band at the weight of ~43kD. In addition the band is responsive to stimuli consistent with phosphorylation of CREB.
- Submitted by
- Kevin Struhl
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG004558