ENCAB000AFJ
Antibody against Homo sapiens RCOR1
Homo sapiens
at least one cell type or tissue
awaiting characterization
- Status
- released
- Source (vendor)
- Abcam
- Product ID
- ab24166
- Lot ID
- 834865
- Characterized targets
- RCOR1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Antigen description
- Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human CoREST. Note: This antibody has since been discontinued. Full product information is attached as part of validation document.
- External resources
Characterizations
RCOR1 (Homo sapiens)
Method: ChIP-seq comparison
not reviewed
- Caption
- Because the overlap of the top 40% of peaks from datasets derived from antibodies sc30189 and ab24166 (compared using standard ENCODE scoring methods) exceeds the recommended threshold of 80%, both antibodies meet this criterion for validation.
- Submitted by
- Michael Snyder
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG004558
RCOR1 (Homo sapiens)
Method: immunoblot
not reviewed
- Caption
- We observe a single band in Western blots on cell lines K562, HeLa S3, and HepG2 that migrates slightly slower than the predicted size (~53kD) would suggest. This has been previously observed (see product literature for this and other commercial antibodies, as well as the Western blot assays in our characterization document for ab24166 against COREST). This band, but not the observed weaker bands, is specifically immunoprecipitated by sc-30189. Based on this, sc-30189 is validated by this criterion.
- Submitted by
- Michael Snyder
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG004558