1. Antibodies
  2. Homo sapiens
  3. CHD7

ENCAB000AFI

Alternate accession: ENCAB433VED

Antibody against Homo sapiens CHD7

Homo sapiens
HeLa, K562, GM12878
partially characterized
Homo sapiens
HepG2, endothelial cell of umbilical vein
not characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A301-223A
Lot ID
1
Characterized targets
CHD7 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
bradley-bernstein:PchAb 357, michael-snyder:AS-1570

Characterizations

CHD7 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
K562 whole cell lysate was immunoprecipitated using primary antibody, and the IP fraction along side a whole cell lysate were loaded on a CriterionXT gel and separated. After separation, the samples were transferred using a wet transfer. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed. Band of expected size visualized representing strongest signal in the lane.We assume that since one of the isoforms is relatively big, it goes through degradation and therefore we get more bands in unexpected areas.
Reviewer comment
No IgG
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
CHD7 (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
Nuclear lysates from K562 (10ug), HepG2 (10ug), GM12878 (7ug), HUVEC (10ug), were loaded into a 3-8% Tris-acetate gel in 1X TA running buffer. After separation, the samples were transferred to a PVDF membrane using wet transfer. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Multiple bands can be detected at expected sizes (~360kDa,130kDa and 100kDa). We assume that since one of the isoforms is relatively big, it goes through degradation and therefore we get more bands in unexpected areas.
Submitter comment
335 kDa according to genecards
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
CHD7 (Homo sapiens)
Method: ChIP-string comparison
Attachment PDF Icon
not reviewed
Caption
Antibody was validated by use of correlation analysis of ChIP-String data obtained using this antibody together with diverse other antibodies
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad
CHD7 (Homo sapiens)
HeLa
Method: immunoblot
Attachment PDF Icon
compliant
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad