ENCAB000AEV

Alternate accessions: ENCAB915AHG, ENCAB781LKK

Antibody against Homo sapiens CBX2

Homo sapiens
HepG2, A549, K562, SKNSH
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
H1-hESC, GM12878
not characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A302-524A
Lot ID
1
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Immunogen Between 482 and 532.
Aliases
bradley-bernstein:PchAb 165, michael-snyder:AS-1534

Characterizations

CBX2 (Homo sapiens)
Method: ChIP-seq comparison
compliant
Caption
This validation relies on the use of antibodies to chromatin regulators in K562 cells, and demonstrates highly similar patterns of enrichment are obtained with each antibody. The first track shown used an antibody to CBX2 (PchAb 165, ENCAB000AEV), and the second track shown used an antibody to CBX8 (PchAb 159, ENCAB000AEX). The third track shows data of a functionally related histone modification (H3K27me3) previously characterized to Encode standards (PchAb 67-V, ENCAB000ASB) – the use of this data is meant to act as a reference for the location in the genome rather than to quantitate to the track-track comparison between CBX2 and CBX8.
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad
CBX2 (Homo sapiens)
A549H1-hESC
Method: immunoblot
Attachment from submitter
compliant
Caption
Nuclear lysates from A549 (10ug) and H1 (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Bands were detected at the expected size (56kDa) for A549 and outside of the expected size for both A549 and H1.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
CBX2 (Homo sapiens)
K562HepG2GM12878SKNSH
Method: immunoblot
Attachment from submitter
compliant
Caption
Nuclear lysates from K562 (10ug), HepG2 (7ug), GM12878 (7ug), SK-N-SH (6ug), were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. a band was detected at expected size in all cell types (~70kDa).
Reviewer comment
Only SKNSH is compliant. MW is actually 56kDa not 70. need to fix arrow and expected size
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
CBX2 (Homo sapiens)
Method: ChIP-string comparison
Attachment from submitter
not reviewed
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad
CBX2 (Homo sapiens)
Method: immunoblot
Attachment from submitter
not reviewed
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad