1. Antibodies
  2. Homo sapiens
  3. MYB

ENCAB000AES

Antibody against Homo sapiens MYB

Homo sapiens
K562, GM12878
characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-7874
Lot ID
J2809
Characterized targets
MYB (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Isotype
IgG
Antigen description
Epitope corresponding to amino acids 500-640 mapping at the C-terminus of c-Myb of human origin. Antibody Target: MYB

Characterizations

MYB (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878 using the antibody sc-7874x. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 72.341.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
MYB (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation of sc-7874 (c-Myb) in cell line K562. Lane 1: Input lysate from cell line K562. Lane 2: Bound material from IP with sc-7874. Lane 3: Bound material from IP using non-specific IgG. This band was taken for mass spectrometry. Expected size: ~72 kD
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
Download
 c-Myb_IP.png
MYB (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
not compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 whole cell lysates using sc-7874, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGE Bis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was silver-stained, gel fragments corresponding to the bands indicated were excised and destained using the SilverSNAP Stain for Mass Spectrometry (Pierce). Then proteins were treated with trypsin using the in-gel digestion method. Digested proteins were analyzed on a LTQ-Orbitrap (Thermo Scientific) b the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (protein false discovery rate <1%, 2 peptides per protein minimum).
Submitter comment
The factor of interest is not within the top 20 detected as ranked by enrichment over IP.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
Download
 cMyb_final.pdf
MYB (Homo sapiens)
Method: motif enrichment
Attachment PDF Icon
compliant
Caption
The motif for target MYB is represented by the attached position weight matrix (PWM) derived from ENCFF351VHSX. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.978051744848 Global enrichment Z-score: 4.50959977272 Positional bias Z-score: 2.34297998098 Peak rank bias Z-score: 1.65116580341 Enrichment rank: 1.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford