ENCAB000AEN
Antibody against Homo sapiens BRF2
Homo sapiens
at least one cell type or tissue
awaiting characterization
- Status
- released
- Source (vendor)
- Robert White
- Product ID
- 4295
- Lot ID
- unknown
- Characterized targets
- BRF2 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Antigen description
- keyhole limpet hemocyanin coupled to synthetic peptides VSRSQQRGLRRVRDLC and SDSEIEQYLRTPQEVR corresponding to human Brf2 residues 66-80 and 385-400, respectively
- Antigen sequence
- VSRSQQRGLRRVRDLC and SDSEIEQYLRTPQEVR
- External resources
Characterizations
BRF2 (Homo sapiens)
not reviewed
- Caption
- Brf2 is a component of an alternative form of the Pol III transcription factor TFIIIB, which is involved in the transcription of a small minority group of Pol III- transcribed genes with upstream extragenic promoters. Brf2 is associated with the expected loci. Although Brf2-bound loci recruit high levels of the Pol III subunit Rpc155, these loci are not associated with the more common TFIIIB subunit Brf1. This is exactly the expected result for a component of an alternative TFIIIB subunit. This is illustrated in Figure 3 from: Moqtaderi, Z., Wang, J., Raha, D., White R.J., Snyder M., Weng Z., and Struhl, K. Genomic binding profiles of functionally distinct RNA polymerase III transcription complexes in human cells. Nat. Struct. Mol. Biol. 2010 May;17(5):635-40. Epub 2010 Apr 25.
- Submitted by
- Kevin Struhl
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG004558
BRF2 (Homo sapiens)
not reviewed
- Caption
- Western blotting with this antibody shows a strong band at the appropriate size in the ENCODE cell line GM12878:
- Submitted by
- Kevin Struhl
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG004558