ENCAB000AEN

Antibody against Homo sapiens BRF2

Homo sapiens
at least one cell type or tissue
awaiting characterization
Status
released
Source (vendor)
Robert White
Product ID
4295
Lot ID
unknown
Characterized targets
BRF2 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Antigen description
keyhole limpet hemocyanin coupled to synthetic peptides VSRSQQRGLRRVRDLC and SDSEIEQYLRTPQEVR corresponding to human Brf2 residues 66-80 and 385-400, respectively
Antigen sequence
VSRSQQRGLRRVRDLC and SDSEIEQYLRTPQEVR

Characterizations

BRF2 (Homo sapiens)
Method: ChIP-seq comparison
Attachment from submitter
not reviewed
Caption
Brf2 is a component of an alternative form of the Pol III transcription factor TFIIIB, which is involved in the transcription of a small minority group of Pol III- transcribed genes with upstream extragenic promoters. Brf2 is associated with the expected loci. Although Brf2-bound loci recruit high levels of the Pol III subunit Rpc155, these loci are not associated with the more common TFIIIB subunit Brf1. This is exactly the expected result for a component of an alternative TFIIIB subunit. This is illustrated in Figure 3 from: Moqtaderi, Z., Wang, J., Raha, D., White R.J., Snyder M., Weng Z., and Struhl, K. Genomic binding profiles of functionally distinct RNA polymerase III transcription complexes in human cells. Nat. Struct. Mol. Biol. 2010 May;17(5):635-40. Epub 2010 Apr 25.
Submitted by
Kevin Struhl
Lab
Michael Snyder, Stanford
BRF2 (Homo sapiens)
Method: immunoblot
Attachment from submitter
not reviewed
Caption
Western blotting with this antibody shows a strong band at the appropriate size in the ENCODE cell line GM12878:
Submitted by
Kevin Struhl
Lab
Michael Snyder, Stanford