at least one cell type or tissue
BHLHE40 (Homo sapiens)
- Western blot protocol: Whole cell lysates were immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane using a Bio-Rad Trans-Blot Electrophoretic Transfer system. Standard western blot protocol was used to probe the membrane with the primary antibody (same antibody as used for IP), and an HRP-conjugated secondary antibody and SuperSignal West Femto solution (Thermo Scientific) were used to detect the immunoprecipitated proteins. Figure Legend: BHLHE40 immunoblot: IP-western with sc- 101023 DEC1 antibody in whole cell lysate of GM12878. Heavy chain and light chain of IgG are indicated, and DEC1 band is indicated at ~45 kDa.
- Submitted by
- Richard Myers
- Richard Myers, HAIB