ENCAB000AEJ
Antibody against Homo sapiens BHLHE40
Homo sapiens
at least one cell type or tissue
awaiting characterization
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-101023
- Lot ID
- unknown
- Characterized targets
- BHLHE40 (Homo sapiens)
- Host
- mouse
- Clonality
- monoclonal
- Isotype
- IgG2b
- Antigen description
- Dec-1(S-8) is an IgG2b antibody raised against recombinant DEC1 of human origin
- External resources
Characterizations
BHLHE40 (Homo sapiens)
Method: immunoblot
not reviewed
- Caption
- Western blot protocol: Whole cell lysates were immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane using a Bio-Rad Trans-Blot Electrophoretic Transfer system. Standard western blot protocol was used to probe the membrane with the primary antibody (same antibody as used for IP), and an HRP-conjugated secondary antibody and SuperSignal West Femto solution (Thermo Scientific) were used to detect the immunoprecipitated proteins. Figure Legend: BHLHE40 immunoblot: IP-western with sc- 101023 DEC1 antibody in whole cell lysate of GM12878. Heavy chain and light chain of IgG are indicated, and DEC1 band is indicated at ~45 kDa.
- Submitted by
- Richard Myers
- Lab
- Richard Myers, HAIB
- Grant
- U54HG004576