ENCAB000AEH
Antibody against Homo sapiens BCLAF1
Homo sapiens
at least one cell type or tissue
awaiting characterization
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-101388
- Lot ID
- unknown
- Characterized targets
- BCLAF1 (Homo sapiens)
- Host
- mouse
- Clonality
- monoclonal
- Isotype
- IgG
- Antigen description
- Raised against a synthetic peptide corresponding to a.a. 900-909 of BCLAF1 of human origin.
- External resources
Characterizations
BCLAF1 (Homo sapiens)
Method: immunoblot
not reviewed
- Caption
- For an antibody to meet ENCODE validation standards, a single band of the predicted size, or a band of no less than half the total signal, must be detected in a lane on a Western blot
- Submitted by
- Richard Myers
- Lab
- Richard Myers, HAIB
- Grant
- U54HG004576
BCLAF1 (Homo sapiens)
Method: immunoblot
not reviewed
- Caption
- Whole cell lysates were immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed on an Invitrogen BenchPro 4100 system. Visualization was achieved using SuperSignal West Femto solution (Thermo Scientific).
- Submitted by
- Richard Myers
- Lab
- Richard Myers, HAIB
- Grant
- U54HG004576