ENCAB000AEG
Antibody against Homo sapiens BCL3
Homo sapiens
at least one cell type or tissue
awaiting characterization
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-185
- Lot ID
- unknown
- Characterized targets
- BCL3 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Antigen description
- Bcl-3(C-14) is an affinity purified antibody Raised against a peptide mapping at the C-terminus of Bcl-3 of human origin.
- External resources
Characterizations
BCL3 (Homo sapiens)
Method: immunoblot
not reviewed
- Caption
- Western blot protocol: Whole cell lysates were immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed on an Invitrogen BenchPro 4100 system. Visualization was achieved using SuperSignal West Femto solution (Thermo Scientific). Figure Legend: BCL3 immunoblot: IP-western with sc-185 BCL3 antibody in whole cell lysates (WCL) of K562, GM12878, and HepG2. Heavy chain of IgG is indicated, and BCL3 band is indicated at ~60 kDa.
- Submitted by
- Richard Myers
- Lab
- Richard Myers, HAIB
- Grant
- U54HG004576
- Download
- human_BCl3_C-14_validation_Myers.pdf