1. Antibodies
  2. Homo sapiens
  3. ATF3

ENCAB000ADZ

Antibody against Homo sapiens ATF3

Homo sapiens
GM12878, K562, HeLa-S3
characterized to standards
Homo sapiens
liver
characterized to standards with exemption
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-188
Lot ID
J2209
Characterized targets
ATF3 (Homo sapiens)
Lot ID aliases
E1406
Host
rabbit
Clonality
polyclonal
Isotype
IgG
Antigen description
Epitope mapping at the C-terminus of ATF3 of human origin.

Characterizations

ATF3 (Homo sapiens)
Method: motif enrichment
Attachment PDF Icon
compliant
Caption
The motif for target ATF3 is represented by the attached position weight matrix (PWM) derived from files ENCFF025OSU and ENCFF776BLV. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab) using known motifs (http://compbio.mit.edu/encode-motifs/) and previously published ChIP-seq data (http://www.broadinstitute.org/~zzhang/motifpipeline/data/TrainSetInfo.txt). The accept probability score of the given transcription factor was calculated using a Bayesian approach. This analysis also includes three motif enrichment scores, computed by overlapping the motif instances with the given ChIP-seq peak locations. For more information on the underlying statistical methods, please see the attached document. From ENCFF025OSU: Accept probability score: 0.644794825464, Global enrichment Z-score: 4.73112472057, Positional bias Z-score: 3.01630902999, Peak rank bias Z-score: 1.59065491152. From ENCFF776BLV: Accept probability score: 0.644794825464, Global enrichment Z-score: 5.40407532093, Positional bias Z-score: 3.48071737029, Peak rank bias Z-score: 1.8842364848, Enrichment rank: 4.0.
Submitted by
Aditi Narayanan
Lab
Richard Myers, HAIB
Download
 ENCAB000ADZ.pdf
ATF3 (Homo sapiens)
GM12878GM12878K562HeLa-S3
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
ATF3 immunoblot: IP-western with sc-188 ATF3 antibody in nuclear lysate (NE) or whole cell lysates (WCL) of GM12878, K562, and HeLa. Heavy chain of IgG is indicated, and ATF3 band is indicated at ~23 kDa.Whole cell lysates were immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed on an Invitrogen BenchPro 4100 system. Visualization was achieved using SuperSignal West Femto solution (Thermo Scientific). Expected size: ~20.5 kDa
Submitted by
Richard Myers
Lab
Richard Myers, HAIB
ATF3 (Homo sapiens)
liver
Method: immunoblot
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
The ENCODE Binding Working Group finds for some valuable tissues that recreating a primary on well characterized antibodies is not cost effective. Therefore, they allow exemption from standards for these tissues.
Submitter comment
The lab is asking for an exemption for liver cells due to the lack of resource to make a primary characterization for them
Reviewer comment
Exempted by the Feb 29, 2016 antibody review panel
Submitted by
Richard Myers
Lab
Richard Myers, HAIB
Download
 No_tissue.png