RNA-seq of long RNAs (single-end, unstranded)

Alternate accession: ENCPL739VZK

Status
released
Title
RNA-seq of long RNAs (single-end, unstranded)
Assays
RNA-seq, single-cell RNA sequencing assay
Description
Pipeline for single-ended unstranded data bulk RNA-seq data as developed by the ENCODE RNA Working Group. RNA libraries generated from mRNAs (poly-A(+)), rRNA-depleted total RNA, or poly-A(-) populations that are size-selected to be longer than approximately 200 bp may be run through this pipeline. More information regarding uniform pipeline restrictions, references used for this pipeline, and exogenous RNA spike-in controls are described in the pipelines overview document.
Lab
ENCODE Processing Pipeline
Award PI
J. Michael Cherry, Stanford
Pipeline standards
Bulk RNA-seq Data Standards

Pipeline schematic

Documents

pipeline protocol
Description excerpt: Bulk RNA-seq pipeline: Includes overview, references, and spike-ins…
Description
Bulk RNA-seq pipeline: Includes overview, references, and spike-ins information about the pipeline
Submitted by
Kathrina Onate
Lab
J. Michael Cherry, Stanford