Experiment summary for ENCSR997UIM
doi:10.17989/ENCSR997UIM
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- pnt
- Biosample summary
- Drosophila melanogaster strain pnt-MiMIC-GFP whole organism embryo (0-24 hours) genetically modified (insertion) using site-specific recombination targeting D. melanogaster pnt
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic flies expressing pnt-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:pnt-MIMIC-GFP
- Date submitted
- October 22, 2015
- Date released
- July 6, 2016
- Submitter comment
- ENCFF849OOW, ENCFF327LRU, ENCFF710PYG, ENCFF313ZLD were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files.
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 2 | 1 | Drosophila melanogaster strain pnt-MiMIC-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster pnt | ENCBS107ZTL | ENCGM295BTR | ENCAB728YTO | ENCLB323LAY |
| 3 | 1 | Drosophila melanogaster strain pnt-MiMIC-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster pnt | ENCBS134TEE | ENCGM295BTR | ENCAB728YTO | ENCLB070RIA |
| 1 | 1 | Drosophila melanogaster strain pnt-MiMIC-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster pnt | ENCBS778XPF | ENCGM295BTR | ENCAB728YTO | ENCLB775LZT |