Experiment summary for ENCSR948EEN
doi:10.17989/ENCSR948EEN
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- dmrt99B
- Biosample summary
- Drosophila melanogaster whole organism embryo (6-16 hours) genetically modified (insertion) using site-specific recombination targeting D. melanogaster dmrt99B
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic flies expressing dmrt99B-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:dmrt99B-GFP
- Date submitted
- December 7, 2018
- Date released
- December 21, 2018
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 4 | 1 | Drosophila melanogaster mixed sex embryo (6-16 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster dmrt99B | ENCBS402SFN | ENCGM079VNG | ENCAB728YTO | ENCLB193UJR |
| 3 | 1 | Drosophila melanogaster mixed sex embryo (6-16 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster dmrt99B | ENCBS126LAY | ENCGM079VNG | ENCAB728YTO | ENCLB840RBX |