Experiment summary for ENCSR946EAE

doi:10.17989/ENCSR946EAE

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
pb
Biosample summary
Drosophila melanogaster strain pb-GFP whole organism embryo (0-24 hours) genetically modified (insertion) using site-specific recombination targeting D. melanogaster pb
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing pb-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:pb-GFP
Date submitted
July 24, 2015
Date released
July 6, 2016
Submitter comment
ENCFF639KRU, ENCFF062WAS, ENCFF012JQZ, ENCFF151BIE were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
31Drosophila melanogaster strain pb-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster pbENCBS939ITPENCGM362DUXENCAB728YTOENCLB128JOX
21Drosophila melanogaster strain pb-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster pbENCBS900MGRENCGM362DUXENCAB728YTOENCLB645QWH
11Drosophila melanogaster strain pb-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster pbENCBS435UGZENCGM362DUXENCAB728YTOENCLB548BXR