1. Experiments
  2. ChIP-seq
  3. Drosophila melanogaster
  4. whole organism

Experiment summary for ENCSR872ZXK

doi:10.17989/ENCSR872ZXK

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
HLH54F
Biosample summary
Drosophila melanogaster strain HLH54F-GFP whole organism embryo (0-24 hours) genetically modified (insertion) using site-specific recombination targeting D. melanogaster HLH54F
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing HLH54F-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:HLH54F-GFP
Date submitted
July 24, 2015
Date released
July 6, 2016
Submitter comment
ENCFF179HQL, ENCFF979YQK were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21Drosophila melanogaster strain HLH54F-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster HLH54FENCBS463WWZENCGM340GXJENCAB728YTOENCLB929WMH
31Drosophila melanogaster strain HLH54F-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster HLH54FENCBS448EGRENCGM340GXJENCAB728YTOENCLB246KIX
11Drosophila melanogaster strain HLH54F-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster HLH54FENCBS004OUOENCGM340GXJENCAB728YTOENCLB250QSD

Files

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Output type
Replicates

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