Experiment summary for ENCSR851UBK
doi:10.17989/ENCSR851UBK
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- dac
- Biosample summary
- Drosophila melanogaster strain dac-GFP whole organism prepupa (48 days) genetically modified (insertion) using site-specific recombination targeting D. melanogaster dac
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic flies expressing dac-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:dac-GFP
- Date submitted
- December 21, 2016
- Date released
- January 11, 2017
- Submitter comment
- ENCFF186MCZ, ENCFF070IWL were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Drosophila melanogaster strain dac-GFP mixed sex prepupa (48 days) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster dac | ENCBS026OWX | ENCGM980RWZ | ENCAB728YTO | ENCLB974BHR |
| 3 | 1 | Drosophila melanogaster strain dac-GFP mixed sex prepupa (48 days) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster dac | ENCBS362JKV | ENCGM980RWZ | ENCAB728YTO | ENCLB568CFM |