Experiment summary for ENCSR811GFT

doi:10.17989/ENCSR811GFT

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
Ets65A
Biosample summary
Drosophila melanogaster strain ets3-GFP whole organism embryo (0-24 hours) genetically modified (insertion) using site-specific recombination targeting D. melanogaster Ets65A
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing Ets65A-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:Ets65A-GFP
Date submitted
July 23, 2015
Date released
July 6, 2016
Submitter comment
ENCFF446GEC, ENCFF009ATE were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11Drosophila melanogaster strain ets3-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Ets65AENCBS533ZIXENCGM323DQEENCAB728YTOENCLB954FUK
21Drosophila melanogaster strain ets3-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Ets65AENCBS479BMEENCGM323DQEENCAB728YTOENCLB529MOB
31Drosophila melanogaster strain ets3-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Ets65AENCBS747BIYENCGM323DQEENCAB728YTOENCLB098XAV