Experiment summary for ENCSR731HHO

doi:10.17989/ENCSR731HHO

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
Sox102F
Biosample summary
Drosophila melanogaster strain Sox102F-GFP whole organism prepupa at white prepupa stage genetically modified (insertion) using site-specific recombination targeting D. melanogaster Sox102F
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing Sox102F-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:Sox102F-GFP
Date submitted
July 24, 2015
Date released
July 6, 2016
Submitter comment
ENCFF818IOQ, ENCFF855QBV, ENCFF457EVR, ENCFF007ANM were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
41Drosophila melanogaster strain Sox102F-GFP mixed sex prepupa at white prepupa stage whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Sox102FENCBS542APEENCGM523XMLENCAB728YTOENCLB968ZIE
11Drosophila melanogaster strain Sox102F-GFP mixed sex prepupa at white prepupa stage whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Sox102FENCBS548EBZENCGM523XMLENCAB728YTOENCLB468ZUU