Experiment summary for ENCSR682NHG

doi:10.17989/ENCSR682NHG

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
NC2β
Biosample summary
Drosophila melanogaster strain NC2beta-GFP whole organism embryo (0-24 hours) genetically modified (insertion) using site-specific recombination targeting D. melanogaster NC2beta
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing NC2β-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:NC2beta-GFP
Date submitted
July 24, 2015
Date released
September 29, 2016
Submitter comment
ENCFF772YMO and ENCFF285LMB were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11Drosophila melanogaster strain NC2beta-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster NC2betaENCBS918HZMENCGM005RNZENCAB728YTOENCLB311KOH
31Drosophila melanogaster strain NC2beta-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster NC2betaENCBS924LCGENCGM005RNZENCAB728YTOENCLB919VQX