Experiment summary for ENCSR603RPC
doi:10.17989/ENCSR603RPC
Summary
- Status
- released
- Assay
- small RNA-seq
- Biosample summary
- Homo sapiens bipolar neuron originated from GM23338 treated with 0.5 μg/mL doxycycline hyclate for 4 days
- Biosample Type
- in vitro differentiated cells
- Replication type
- isogenic
- Description
- The libraries contained in this experiment come from independent growths of bipolar neurons that were differentiated from induced pluripotent stem cells. They are stranded SE101 Illumina Hi-Seq RNA-Seq libraries from rRNA-depleted Total RNA < 200 nucleotides in size that was pre-treated with TAP prior to cloning.
- Treatments
- Tobacco Acid Pyrophosphatase for 1 hour, 0.5 μg/mL doxycycline hyclate (CHEBI:34730) for 4 days
- Nucleic acid type
- RNA
- Depleted in
- rRNA
- Size range
- <200
- Fragmentation methods
- see document
- Strand specificity
- Strand-specific (forward)
- Platform
- Illumina HiSeq 2000
Attribution
ENCODE3 project
- Lab
- Thomas Gingeras, CSHL
- Award
- U54HG007004 (Thomas Gingeras, CSHL)
- Project
- ENCODE
- External resources
- References
- Date submitted
- December 13, 2014
- Date released
- December 17, 2014
- Reference Epigenome
- ENCSR954HHV
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
---|---|---|---|---|
1 | 1 | Homo sapiens male adult (53 years) bipolar neuron in vitro differentiated cells originated from GM23338 treated with 0.5 μg/mL doxycycline hyclate for 4 days | ENCBS369AAA | ENCLB056HVD |
2 | 1 | Homo sapiens male adult (53 years) bipolar neuron in vitro differentiated cells originated from GM23338 treated with 0.5 μg/mL doxycycline hyclate for 4 days | ENCBS374AAA | ENCLB395ZGN |