Experiment summary for ENCSR603QIJ
doi:10.17989/ENCSR603QIJ
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- emc
- Biosample summary
- Drosophila melanogaster strain emc-GFP whole organism embryo (0-24 hours) genetically modified (insertion) using site-specific recombination targeting D. melanogaster emc
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic flies expressing emc-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2500
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:emc-GFP
- Date submitted
- July 24, 2015
- Date released
- July 6, 2016
- Submitter comment
- ENCFF669TKL, ENCFF895GEZ, ENCFF139UAF, ENCFF378XNI were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
---|---|---|---|---|---|---|
1 | 1 | Drosophila melanogaster strain emc-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster emc | ENCBS258EJI | ENCGM375ZDM | ENCAB728YTO | ENCLB272UJF |
3 | 1 | Drosophila melanogaster strain emc-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster emc | ENCBS609FGG | ENCGM375ZDM | ENCAB728YTO | ENCLB088EID |