Experiment summary for ENCSR548EHZ
doi:10.17989/ENCSR548EHZ
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- foxo
- Biosample summary
- Drosophila melanogaster strain foxo-GFP whole organism wandering third instar larva genetically modified (insertion) using site-specific recombination targeting D. melanogaster foxo
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic flies expressing foxo-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2500
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:foxo-GFP
- Date submitted
- July 24, 2015
- Date released
- July 6, 2016
- Submitter comment
- ENCFF548MGQ, ENCFF561HHS, ENCFF911UZM, ENCFF156VME were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 5 | 1 | Drosophila melanogaster strain foxo-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster foxo | ENCBS735WIT | ENCGM216ZUS | ENCAB728YTO | ENCLB671QGN |
| 6 | 1 | Drosophila melanogaster strain foxo-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster foxo | ENCBS689DMT | ENCGM216ZUS | ENCAB728YTO | ENCLB777XUP |