1. Experiments
  2. ChIP-seq
  3. Drosophila melanogaster
  4. whole organism

Experiment summary for ENCSR425SCD

doi:10.17989/ENCSR425SCD

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
HmgD
Biosample summary
Drosophila melanogaster strain HmgD-GFP whole organism embryo (0-24 hours) genetically modified (insertion) using site-specific recombination targeting D. melanogaster HmgD
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing HmgD-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:HmgD-GFP
Date submitted
July 24, 2015
Date released
July 6, 2016
Submitter comment
ENCFF779VGU, ENCFF547ZLV, ENCFF515YPY, ENCFF924IBH were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
31Drosophila melanogaster strain HmgD-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster HmgDENCBS970KWQENCGM396EFGENCAB728YTOENCLB439JDM
21Drosophila melanogaster strain HmgD-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster HmgDENCBS287ZALENCGM396EFGENCAB728YTOENCLB121FME
11Drosophila melanogaster strain HmgD-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster HmgDENCBS415MMCENCGM396EFGENCAB728YTOENCLB820JYR

Files

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Output type
Replicates

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