1. Experiments
  2. ChIP-seq
  3. Drosophila melanogaster
  4. whole organism

Experiment summary for ENCSR396LMJ

doi:10.17989/ENCSR396LMJ

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
YL-1
Biosample summary
Drosophila melanogaster strain YL-1-GFP whole organism embryo (0-24 hours) genetically modified (insertion) using site-specific recombination targeting D. melanogaster YL-1
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing YL-1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:YL-1-GFP
Date submitted
December 21, 2016
Date released
January 10, 2017
Submitter comment
ENCFF658ABL, ENCFF728OKB, ENCFF336GLT were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
31Drosophila melanogaster strain YL-1-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster YL-1ENCBS550UXWENCGM639CFKENCAB728YTOENCLB846TZH
21Drosophila melanogaster strain YL-1-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster YL-1ENCBS964EYKENCGM639CFKENCAB728YTOENCLB404JZJ
11Drosophila melanogaster strain YL-1-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster YL-1ENCBS630ETIENCGM639CFKENCAB728YTOENCLB058EHS

Files

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Output type
Replicates

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