Experiment summary for ENCSR365IMJ

doi:10.17989/ENCSR365IMJ

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
dmrt11E
Biosample summary
Drosophila melanogaster whole organism embryo (2-10 hours) genetically modified (insertion) using site-specific recombination targeting D. melanogaster dmrt11E
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing dmrt11E-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:dmrt11E-GFP
Date submitted
December 7, 2018
Date released
December 21, 2018

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
31Drosophila melanogaster mixed sex embryo (2-10 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster dmrt11EENCBS502DCSENCGM994BMBENCAB728YTOENCLB391TFU
11Drosophila melanogaster mixed sex embryo (2-10 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster dmrt11EENCBS828NBZENCGM994BMBENCAB728YTOENCLB964NBK
21Drosophila melanogaster mixed sex embryo (2-10 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster dmrt11EENCBS900ANDENCGM994BMBENCAB728YTOENCLB412DTO