Experiment summary for ENCSR291KUB
doi:10.17989/ENCSR291KUB
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- Rel
- Biosample summary
- Drosophila melanogaster strain Rel-GFP whole organism wandering third instar larva genetically modified (insertion) using site-specific recombination targeting D. melanogaster Rel
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic flies expressing Rel-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2500
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:Rel-GFP
- Date submitted
- July 24, 2015
- Date released
- July 6, 2016
- Submitter comment
- ENCFF707KFY, ENCFF584KZL were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files. This experiment was conducted with C-terminally tagged Rel. The majority of full-length Rel protein is localized to the cytoplasm prior to cleavage, when the N-terminal portion can translocate into the nucleus. We plan on repeating this experiment with an N-terminal GFP tag.
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 2 | 1 | Drosophila melanogaster strain Rel-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Rel | ENCBS079KXG | ENCGM696ISO | ENCAB728YTO | ENCLB146SRJ |
| 3 | 1 | Drosophila melanogaster strain Rel-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Rel | ENCBS853AJH | ENCGM696ISO | ENCAB728YTO | ENCLB916EEP |