Experiment summary for ENCSR240NHL
doi:10.17989/ENCSR240NHL
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- ac
- Biosample summary
- Drosophila melanogaster strain ac-GFP whole organism embryo (0-8 hours) genetically modified (insertion) using site-specific recombination targeting D. melanogaster ac
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic flies expressing ac-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:ac-GFP
- Date submitted
- December 21, 2016
- Date released
- January 10, 2017
- Submitter comment
- ENCFF931VLQ, ENCFF407JJC, ENCFF235MMB, ENCFF541WSZ were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
---|---|---|---|---|---|---|
3 | 1 | Drosophila melanogaster strain ac-GFP mixed sex embryo (0-8 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster ac | ENCBS183GUG | ENCGM377WKQ | ENCAB728YTO | ENCLB644GQN |
1 | 1 | Drosophila melanogaster strain ac-GFP mixed sex embryo (0-8 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster ac | ENCBS854AAQ | ENCGM377WKQ | ENCAB728YTO | ENCLB851WYC |
2 | 1 | Drosophila melanogaster strain ac-GFP mixed sex embryo (0-8 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster ac | ENCBS385CQF | ENCGM377WKQ | ENCAB728YTO | ENCLB972EIQ |