Experiment summary for ENCSR196VPW
doi:10.17989/ENCSR196VPW
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- Hr4
- Biosample summary
- Drosophila melanogaster strain Hr4-GFP whole organism prepupa (48 days) at white prepupa stage genetically modified (insertion) using site-specific recombination targeting D. melanogaster Hr4
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic flies expressing Hr4-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2500
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:Hr4-GFP
- Date submitted
- July 24, 2015
- Date released
- July 6, 2016
- Submitter comment
- ENCFF411KVD, ENCFF940IMV, ENCFF663EOG, ENCFF602MGP were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Drosophila melanogaster strain Hr4-GFP mixed sex prepupa (48 days) at white prepupa stage whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Hr4 | ENCBS933EQR | ENCGM459OKC | ENCAB728YTO | ENCLB733QQW |
| 3 | 1 | Drosophila melanogaster strain Hr4-GFP mixed sex prepupa (48 days) at white prepupa stage whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Hr4 | ENCBS747LOH | ENCGM459OKC | ENCAB728YTO | ENCLB617MCZ |
| 2 | 1 | Drosophila melanogaster strain Hr4-GFP mixed sex prepupa (48 days) at white prepupa stage whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Hr4 | ENCBS158JTI | ENCGM459OKC | ENCAB728YTO | ENCLB078RLY |