Experiment summary for ENCSR165OGT
doi:10.17989/ENCSR165OGT
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- topi
- Biosample summary
- Drosophila melanogaster strain topi-GFP whole organism prepupa at white prepupa stage genetically modified (insertion) using site-specific recombination targeting D. melanogaster topi
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic flies expressing topi-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2500
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:topi-GFP
- Date submitted
- July 24, 2015
- Date released
- July 6, 2016
- Submitter comment
- ENCFF237RZL, ENCFF710MEM, ENCFF594RZB, ENCFF416WTY were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
---|---|---|---|---|---|---|
2 | 1 | Drosophila melanogaster strain topi-GFP mixed sex prepupa at white prepupa stage whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster topi | ENCBS996WJP | ENCGM231WNX | ENCAB728YTO | ENCLB608VHB |
1 | 1 | Drosophila melanogaster strain topi-GFP mixed sex prepupa at white prepupa stage whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster topi | ENCBS350HNE | ENCGM231WNX | ENCAB728YTO | ENCLB887PSI |
3 | 1 | Drosophila melanogaster strain topi-GFP mixed sex prepupa at white prepupa stage whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster topi | ENCBS843QED | ENCGM231WNX | ENCAB728YTO | ENCLB783TOD |