Experiment summary for ENCSR007HUT

doi:10.17989/ENCSR007HUT

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
cyc
Biosample summary
Drosophila melanogaster strain cyc-GFP whole organism prepupa at white prepupa stage genetically modified (insertion) using site-specific recombination targeting D. melanogaster cyc
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing cyc-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:cyc-GFP
Date submitted
July 24, 2015
Date released
July 6, 2016
Submitter comment
ENCFF436BEZ, ENCFF641QOX were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11Drosophila melanogaster strain cyc-GFP mixed sex prepupa at white prepupa stage whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster cycENCBS107UARENCGM427WIYENCAB728YTOENCLB979HBE
31Drosophila melanogaster strain cyc-GFP mixed sex prepupa at white prepupa stage whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster cycENCBS164XLCENCGM427WIYENCAB728YTOENCLB350NOE
21Drosophila melanogaster strain cyc-GFP mixed sex prepupa at white prepupa stage whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster cycENCBS859CWXENCGM427WIYENCAB728YTOENCLB925OFY