ENCBS931TFT / cell line
Summary
- Status
- released
- Term name
- A549
- Term ID
- EFO:0001086
- Summary
- Homo sapiens A549 cell line genetically modified (insertion) using CRISPR targeting H. sapiens NR5A2
- Culture harvest date
- 2018-05-04
- Separated from biosample
- ENCBS723DBL
Attribution
ENCODE4 project
- Lab
- Michael Snyder, Stanford
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Nathaniel Watson
- Source
- Michael Snyder
- Project
- ENCODE
- External resources
- Aliases
- michael-snyder:pB-2578, michael-snyder:pB-eGFP-NR5A2 A549 rep1
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
---|---|---|---|---|---|
ENCGM623TXY | insertion | tagging | CRISPR |
|
Donor information
- Status
- released
- Accession
- ENCDO000AAZ
- Aliases
- encode:donor of A549, bradley-bernstein:Donor of A549 cells, john-stamatoyannopoulos:ATCC_A549
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 58 years
- Sex
- male
- Ethnicity
- European
- External resources
- References
Documents
immunoblot
- Caption
- Immunoprecipitation was performed on nuclear extracts from biosample ENCBS931TFT (A549 eGFP-NR5A2 replicate 1) cells using anti-eGFP antibody. The image shows western blot analysis of input (lane 1), immunoprecipitate (lane 2), and mock immunoprecipitate using IgG (lane 3). Molecular weight standard (Bio-Rad, cat. # 161-0374) contains 10 pre-stained recombinant proteins (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD). The target molecular weight is 90.0 kD as indicated with an arrow.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- UM1HG009442
- Submitter comment
- Upper band is within the 20% size range (90-20%=72).
- Documents
transfection protocol
- Description
- A549 transfection protocol - Version 1 (Replicates split right after transfection, 500 µg/ml G418)
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996