ENCBS843UJX / cell line

Summary

Status
released
Term name
K562
Summary
Homo sapiens K562 cell line genetically modified (insertion) using CRISPR targeting H. sapiens ZNF253
Separated from biosample
ENCBS827HZH

Attribution

ENCODE4 project
Lab
Michael Snyder, Stanford
Award PI
Michael Snyder, Stanford
Submitted by
Tao Wang
Source
ATCC
Project
ENCODE
Aliases
michael-snyder:pB-12108, michael-snyder:pB-eGFP-ZNF253 K562 Rep1

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM287HLBinsertiontaggingCRISPR

Donor information

Status
released
Accession
ENCDO000AAD
Aliases
encode:donor of K562, bradley-bernstein:Donor of K562 cells
Species
Homo sapiens
Life stage
adult
Age
53 years
Sex
female
Health status
chronic myelogenous leukemia (CML)
External resources
References

Documents

immunoblot
Caption excerpt: Immunoprecipitation was performed on nuclear extracts from biosample…
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from biosample ENCBS843UJX (K562 eGFP-ZNF253 replicate 2) cells using anti-eGFP antibody.The image shows western blot analysis of immunoprecipitation. Molecular weight standard (Bio-Rad, cat. # 161-0374) contains 10 pre-stained recombinant proteins (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD).The target molecular weight is indicated.
Submitted by
Tao Wang
Lab
Michael Snyder, Stanford