ENCBS806SBR / cell line
Summary
- Status
- released
- Term name
- SK-N-SH
- Term ID
- EFO:0003072
- Summary
- Homo sapiens SK-N-SH cell line genetically modified (insertion) using CRISPR targeting H. sapiens BNC2 treated with 6 μM all-trans-retinoic acid for 48 hours
- Culture harvest date
- 2018-07-27
- Separated from biosample
- ENCBS858DVM
Attribution
ENCODE4 project
- Lab
- Michael Snyder, Stanford
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Nathaniel Watson
- Source
- Michael Snyder
- Project
- ENCODE
- External resources
- Aliases
- michael-snyder:pB-11117, michael-snyder:pB-eGFP-BNC2 SK-N-SH rep2
Treatment details
- Treatment
- 6 μM all-trans-retinoic acid (CHEBI:15367) for 48 hours
- Type
- chemical
- Purpose
- activation
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
---|---|---|---|---|---|
ENCGM998JVG | insertion | tagging | CRISPR |
|
Donor information
- Status
- released
- Accession
- ENCDO000ABD
- Aliases
- encode:donor of SK-N-SH, bradley-bernstein:Donor of SK-N-SH cells, encode:donor of SH-SY5Y
- Species
- Homo sapiens
- Life stage
- child
- Age
- 4 years
- Sex
- female
- Health status
- Metastatic neuroblastoma from bone marrow
- External resources
- References
Documents
immunoblot
- Caption
- Immunoprecipitation was performed on nuclear extracts from biosample ENCBS806SBR (SK-N-SH eGFP-BNC2 replicate 2) cells using anti-eGFP antibody. The image shows western blot analysis of input (lane 1), immunoprecipitate (lane 2), and mock immunoprecipitate using IgG (lane 3). Molecular weight standard (Bio-Rad, cat. # 161-0374) contains 10 pre-stained recombinant proteins (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD). The target molecular weight is 150.0 kD as indicated with an arrow. Lower size bands which may be due to potential degradation products are marked with asterisks.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- UM1HG009442
- Documents
transfection protocol
- Description
- SK-N-SH transfection protocol - Version 2 (Replicates split right after transfection, 200 µg/ml G418)
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- UM1HG009442