ENCBS673GRR / cell line

Summary

Status
released
Term name
K562
Summary
Homo sapiens K562 cell line genetically modified (insertion) using CRISPR targeting H. sapiens ZNF764
Culture harvest date
2017-06-06
Separated from biosample
ENCBS993QKW

Attribution

ENCODE4 project
Lab
Michael Snyder, Stanford
Award PI
Michael Snyder, Stanford
Submitted by
Nathaniel Watson
Project
ENCODE
Aliases
michael-snyder:pB-3044, michael-snyder:pB-eGFP-ZNF764 K562 rep2

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM650SREinsertiontaggingCRISPR

Donor information

Status
released
Accession
ENCDO000AAD
Aliases
encode:donor of K562, bradley-bernstein:Donor of K562 cells
Species
Homo sapiens
Life stage
adult
Age
53 years
Sex
female
Health status
chronic myelogenous leukemia (CML)
External resources
References

Documents

immunoblot
Caption excerpt: Immunoprecipitation was performed on nuclear extracts from biosample…
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from biosample ENCBS016DSZ (K562 eGFP-ZNF764 replicate 1) cells using anti-eGFP antibody.The image shows western blot analysis of immunoprecipitation. Molecular weight standard (Bio-Rad, cat. # 161-0374) contains 10 pre-stained recombinant proteins (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD).The target molecular weight is indicated.
Submitted by
Jessica Au
Lab
Michael Snyder, Stanford
Submitter comment
DCC submitted redundant biosample characterization based off of this biosample-characterizations/45697c2e-7879-4c4f-87af-80ba0d2c0816/ object
growth protocol
Description: Cell growth protocol for K562 cells (version 1).
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
transfection protocol
Description excerpt: K562 transfection protocol - Version 1 (Replicates split at T-75 stage, 50 and…
Description
K562 transfection protocol - Version 1 (Replicates split at T-75 stage, 50 and 500 µg/ml G418)
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford