ENCBS089ARY / cell line

Status: released
Term name: K562
Term ID: EFO:0002067
Summary: Homo sapiens K562 cell line genetically modified (insertion) using CRISPR targeting H. sapiens SHOX2
Culture harvest date: 2018-06-26
Passage number: 6
Separated from biosample: ENCBS517QZD

Accession	Category	Purpose	Method	Nucleic acid delivery method	Site
ENCGM563DKM	insertion	tagging	CRISPR		Target SHOX2-human

Caption excerpt: Immunoprecipitation was performed on nuclear extracts from biosample…

Caption: Immunoprecipitation was performed on nuclear extracts from biosample ENCBS089ARY (K562 eGFP-SHOX2 replicate 2) cells using anti-eGFP antibody.The image shows western blot analysis of immunoprecipitation. Molecular weight standard (Bio-Rad, cat. # 161-0374) contains 10 pre-stained recombinant proteins (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD).The target molecular weight is indicated. Band pattern similar to the one seen in https://www.scbt.com/p/shox2-antibody-jk-6e
Submitted by: Tao Wang
Lab: Michael Snyder, Stanford
Grant: UM1HG009442

Description: Cell growth protocol for K562 cells (version 1).

Description excerpt: K562 transfection protocol - Version 2 (Replicates split right after…

Description: K562 transfection protocol - Version 2 (Replicates split right after transfection, 50 and 500 µg/ml G418)
Submitted by: Nathaniel Watson
Lab: Michael Snyder, Stanford
Grant: UM1HG009442