ENCAB796NDK
Antibody against Homo sapiens ARID1B
Homo sapiens
K562
characterized to standards with exemption
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A301-046A
- Lot ID
- 2
- Characterized targets
- ARID1B (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Aliases
- michael-snyder:AS-1484
- External resources
Characterizations
ARID1B (Homo sapiens)
compliant
- Caption
- IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody A301-046A, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
- Submitter comment
- ARID1B, SMARCE1, SMARCD2, SMARCC1, ACTL6A, SMARCD1, SMARCB1 and SMARCC2 are components of SWI/SNF complex, http://www.genecards.org/cgi-bin/carddisp.pl?gene=ARID1B&keywords=arid1b. ARID1B and DPF2 have interaction, https://thebiogrid.org/121559/summary/homo-sapiens/arid1b.html.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- ARID1B_A301-046A final.pdf
ARID1B (Homo sapiens)
K562
not compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody A301-046A. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 236.123
- Reviewer comment
- bad IgG smear, multiple bands
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- expt1083_6-ARID1B-A301-046A.jpg
ARID1B (Homo sapiens)
K562
exempt from standards
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody A301-046A. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 236.123.
- Submitter comment
- Multiple immunoreactive bands (some outside of the 20% allowed deviation) were detected and analyzed by mass spec.
- Reviewer comment
- ARID1B was detected in band B (at unexpected size) by mass spec
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- MS1040_1_ARID1B-A301-046A_2bands.jpg